Journal: iScience
Article Title: Suppression of TREX1 deficiency-induced cellular senescence and interferonopathies by inhibition of DNA damage response
doi: 10.1016/j.isci.2023.107090
Figure Lengend Snippet: BML-277 alleviates lupus-like phenotypes in Trex1 -/- mice Trex1 −/− mice 6 to 8 weeks of age were given a daily dose of 1 mg/kg BML-277 or solvent (5% DMSO 10% PEG300 2.5% Tween-80 in PBS) control by intraperitoneal injection for 4 weeks (n > 6 per group). (A) Representative treated mouse body size. (B) Body weight in treated and untreated WT and Trex1 −/− mice. (C) Representative treated mouse spleen. (D) Spleen weight in treated and untreated WT and Trex1 −/− mice. (E) Representative plots of H&E analyses of heart, kidney, lung, and skeletal muscle. (F–I) Levels of autoantibodies in mouse serum determined by ELISA, (F) anti-dsDNA IgG1, (G) anti-ssDNA IgG, (H) anti-ANA Total Ig, and (I) anti-nRNP IgG. Data represent mean ± S.E.M. of at least 3 independent experiments. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001, p values calculated using two-way ANOVA with Bonferroni’s correction (B, D, F–I).
Article Snippet: Mouse Anti-Nuclear Antigens (ANA/ENA) Ig’s ELISA Kit, 96 tests, Quantitative , Alpha Diagnostics International , 5210.
Techniques: Solvent, Control, Injection, Enzyme-linked Immunosorbent Assay